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关注:1
2013-05-23 12:21
求翻译:MCF-7 cells (56105) were plated in a 25 cm2 cell culture flask. After 24 h, the cells were treated with PBS vehicle alone or PAP-3 for indicated time, and then the cells were collected and lysed with 0.1 mL of cold lysis buffer (150 mM NaCl, 50 mM of pH 7.4 Tris, 1 mM EDTA, 1% Triton X-100, 0.5% SDS, 0.01% PMSF).是什么意思? 待解决
悬赏分:1
- 离问题结束还有
MCF-7 cells (56105) were plated in a 25 cm2 cell culture flask. After 24 h, the cells were treated with PBS vehicle alone or PAP-3 for indicated time, and then the cells were collected and lysed with 0.1 mL of cold lysis buffer (150 mM NaCl, 50 mM of pH 7.4 Tris, 1 mM EDTA, 1% Triton X-100, 0.5% SDS, 0.01% PMSF).
问题补充: |
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2013-05-23 12:21:38
MCF-7细胞(56105)接种于25 cm2的细胞培养瓶中。 24小时后,将细胞用pbs的车辆单独或子宫颈-3指定的时间,然后收集细胞并裂解用0.1ml冷的裂解缓冲液(150 mM氯化钠,50毫米,1mM EDTA的溶液,pH值7.4三,1%的Triton X-100,0.5%SDS,0.01%PMSF)。
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2013-05-23 12:23:18
MCF-7细胞(56105)在一25 cm2被镀了细胞培养烧瓶。在24个h以后,细胞对待了与PBS被表明的时间的单独车或PAP-3,细胞然后收集了并且溶解了与0.1 mL冷的病势渐退缓冲(150 mM NaCl, 50 mM酸碱度7.4 Tris, 1 mM乙二胺四乙酸, 1%氚核X-100, 0.5% SDS, 0.01% PMSF)。
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2013-05-23 12:24:58
正在翻译,请等待...
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2013-05-23 12:26:38
Mcf-7 细胞 (56105) 被镀在 25 cm2 细胞培养瓶中。后 24 h,单元格对待了以单独的 PBS 车或 PAP-3 为表示时间,然后单元格被收集,并与 0.1 毫升的冷裂解缓冲液进行裂解。 (150 毫米氯化钠,50 毫米的 pH 7.4 招聘,1 毫米乙二胺四乙酸,1%Triton X-100,0.5%SDS 0.01 %pmsf)。
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2013-05-23 12:28:18
正在翻译,请等待...
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