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关注:1
2013-05-23 12:21
求翻译:Methods: (1) Construction of expression vector: Planed to construct three different expression vectors,[PET-22b(+)-LSECtin- CRD、PGEX-6p-1-LSECtin-CRD and PGEX-6p-1-LSECtin] Primers of DNA fragments (LSECtin and LSECtin-CRD) were designed and synthesized according to the purpose of the experiment, products of PCR amplif是什么意思?![]() ![]() Methods: (1) Construction of expression vector: Planed to construct three different expression vectors,[PET-22b(+)-LSECtin- CRD、PGEX-6p-1-LSECtin-CRD and PGEX-6p-1-LSECtin] Primers of DNA fragments (LSECtin and LSECtin-CRD) were designed and synthesized according to the purpose of the experiment, products of PCR amplif
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2013-05-23 12:21:38
正在翻译,请等待...
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2013-05-23 12:23:18
方法:
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2013-05-23 12:24:58
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2013-05-23 12:26:38
方法: (1) 表达载体的构建: 构建三个不同表达载体,刨光 [宠物-22b (+)-LSECtin-CRD、PGEX-6 p-1-LSECtin-CRD 和 PGEX-6 p-1-LSECtin] 引物的 DNA 片段 (LSECtin 和 LSECtin CRD) 设计并合成根据实验的目的、 确定产品的 PCR 扩增了由琼脂糖凝胶,鉴定凝胶回收做好后,双消化的 PCR 片段被证实为正确然后获取的外源 DNA 的凝胶回收净化小片段。之后,DNA 片段被克隆成原核表达载体,转变为扩大文化主管细胞 生署 5α 的主机,表达载体 [PET-22b(+) 和 PGEX-6 p-1] 是分离和纯化
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2013-05-23 12:28:18
正在翻译,请等待...
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