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关注：1
2013-05-23 12:21
求翻译：b -Glucosidase activity was determined by assaying the release of p -nitrophenol ( pNP) at 430 nm from a reaction mixture containing 1 ml p-nitrophenyl glucopyranoside (p NPG) (1 mM), 1.8 ml acetate buffer and 0.2 ml suitably diluted enzyme, incubated at 50 C for 30 min是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
b -Glucosidase activity was determined by assaying the release of p -nitrophenol ( pNP) at 430 nm from a reaction mixture containing 1 ml p-nitrophenyl glucopyranoside (p NPG) (1 mM), 1.8 ml acetate buffer and 0.2 ml suitably diluted enzyme, incubated at 50 C for 30 min
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2013-05-23 12:21:38
β-葡萄糖苷酶的活性是通过在从含有1ml对 - 硝基苯基吡喃葡萄糖苷（对NPG） （1毫摩尔）的反应混合物430nm处测定对硝基苯酚的释放（PNP）确定，1.8毫升的醋酸缓冲液和0.2ml适当稀释的酶
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2013-05-23 12:23:18
正在翻译，请等待...
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2013-05-23 12:24:58
b -葡糖苷酶活动取决于检验p -亚硝基苯酚pNP发行 ( ) 在430毫微米从包含1机器语言磷硝基苯酚glucopyranoside p NPG的 (反应混合物) (1毫米)、1.8机器语言醋酸盐缓冲和0.2机器语言适当地被稀释的酵素，被孵化在50 C 30分钟
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2013-05-23 12:26:38
b-葡萄糖苷酶活性由测定释放的 p-硝基苯酚 (pNP) 在 430 从含有硝基苯吡喃葡萄糖苷 1 毫升 (p NPG) 的反应混合物毫微米 （1 毫米），1.8 毫升醋酸缓冲和 0.2 毫升适当稀释的酶、 孵化在 50 C 30 分钟
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2013-05-23 12:28:18
b -Glucosidase activity was determined by assaying the release of p -nitrophenol ( pNP) at 430 nm from a reaction mixture containing 1 ml p-nitrophenyl glucopyranoside (p NPG) (1 mM), 1.8 ml acetate buffer and 0.2 ml suitably diluted enzyme, incubated at 50 C for 30 min
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