求翻译：After centrifuging at 12,000 9 g for 3 minutes at 4C, 20 lL of supernatant was mixed with 100 lL PNPP substrate and incubated for 30 minutes at 37C.是什么意思？

待解决

悬赏分：1 - 离问题结束还有

After centrifuging at 12,000 9 g for 3 minutes at 4C, 20 lL of supernatant was mixed with 100 lL PNPP substrate and incubated for 30 minutes at 37C.

问题补充：

匿名	2013-05-23 12:21:38 之后在12,000rpm 9克3分钟离心在4 C，上清液20为1L混合，用100为1L PNPP底物，并在37下温育30分钟， 4 C 。

匿名	2013-05-23 12:23:18 正在翻译，请等待...

匿名	2013-05-23 12:24:58 在分离在12,000 9 g以后在3分钟在4 C， 20 lL上层清液与100 lL PNPP基体混合了并且被孵化了在30分钟在37 C。

匿名	2013-05-23 12:26:38 后 3 分钟在 4 ° C 20 12,000 9 g 离心上清液会被混入 100 lL PNPP 衬底和培养了 37 ℃ 30 分钟

匿名	2013-05-23 12:28:18 After centrifuging at 12,000 9 g for 3 minutes at 4C, 20 lL of supernatant was mixed with 100 lL PNPP substrate and incubated for 30 minutes at 37C.

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