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关注：1
2013-05-23 12:21
求翻译：Twenty-four hours after transfection, cells were collected in lysis buffer and 7 mg of transfected protein lysate was incubated with 4 mg of HA antibody and subsequently bound to Protein G Sepharose beads. To increase levels of RIP1 acetylation, 10 mM AGK2 was added for the last 12 h before cell collection是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
Twenty-four hours after transfection, cells were collected in lysis buffer and 7 mg of transfected protein lysate was incubated with 4 mg of HA antibody and subsequently bound to Protein G Sepharose beads. To increase levels of RIP1 acetylation, 10 mM AGK2 was added for the last 12 h before cell collection
问题补充： |
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2013-05-23 12:21:38
转染后24小时，收集细胞在裂解缓冲液和7毫克染蛋白质裂解物孵育4毫克HA抗体，并随后结合到蛋白质G琼脂糖珠。
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2013-05-23 12:23:18
0-0小时后transfection、单元格中所收集的lysis缓冲区和7mg的transfected lysate蛋白质是孵化与医管局的4mG抗体和随后绑定蛋白G sepharose颗粒。 增加各级acetylation RIP1,10毫米agk2添加在过去的12小时采集
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2013-05-23 12:24:58
二十四个小时在transfection以后，细胞在病势渐退缓冲收集了，并且7毫克transfected蛋白质lysate孵化了与4 HA抗体毫克和随后跳起对蛋白质G琼脂糖小珠。 要增加RIP1乙酰化作用的水平， 10毫米AGK2为最后12 h增加在细胞汇集之前
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2013-05-23 12:26:38
正在翻译，请等待...
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2013-05-23 12:28:18
正在翻译，请等待...
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