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关注：1
2013-05-23 12:21
求翻译：When biotinylating proteins in solution, excess non-reacted biotin and reaction byproducts are easily removed by size exclusion using either desalting columns or dialysis. A 10mL desalting column is best suited for processing reactions involving 1-10mg of protein in approximately 0.5-2mL. For smaller protein amounts or是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
When biotinylating proteins in solution, excess non-reacted biotin and reaction byproducts are easily removed by size exclusion using either desalting columns or dialysis. A 10mL desalting column is best suited for processing reactions involving 1-10mg of protein in approximately 0.5-2mL. For smaller protein amounts or
问题补充： |
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2013-05-23 12:21:38
正在翻译，请等待...
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2013-05-23 12:23:18
当biotinylating蛋白质的解决方案,超过非生物素和反应反应副产品轻松移除大小排斥使用脱盐列或透析。 10毫升海水化淡的列是最适合处理反应,牵涉1毫克的蛋白质,约为0.5ml。 蛋白质数额较小卷或反应,反应和随后biotinylation交换缓冲区可以在一个单一热科学幻灯片-A-lyzer迷你透析装置。
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2013-05-23 12:24:58
When biotinylating proteins in solution, excess non-reacted biotin and reaction byproducts are easily removed by size exclusion using either desalting columns or dialysis. A 10mL desalting column is best suited for processing reactions involving 1-10mg of protein in approximately 0.5-2mL. 为更小的蛋白质数额或
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2013-05-23 12:26:38
当 biotinylating 蛋白在解决方案、 过剩非反应生物素和反应副产品是容易删除通过使用脱盐列或透析的尺寸排阻。一个 10 毫升的脱盐柱是最适合处理反应涉及 1-10 毫克的蛋白质约 0.5-2 毫升。对于少量蛋白质或反应卷，生物素化反应和随后的缓冲区交换可以执行单一的热科学幻灯片-A-Lyzer 迷你透析单位。对于反应卷太大，柱脱盐处理，拆分两列之间的样品或使用适当热科学幻灯片-A-Lyzer 透析卡带。处理小卷 (即 10-150μL) 的多肽和其他低分子量分子，热科学皮尔斯 C18 旋转列 (产品号: 89870 或 89873) 可用于。
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2013-05-23 12:28:18
正在翻译，请等待...
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