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关注：1
2013-05-23 12:21
求翻译：The ArrayPlate assay was performed as previously described.(12;13) Briefly, following lysis of the tissue sections, 50-mer probes specific for the genes of interest were incubated with the samples, forming specific probe-mRNA duplexes, then unhybridized probes were digested by S1 Nuclease, followed by alkaline hydroly是什么意思？ 待解决
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The ArrayPlate assay was performed as previously described.(12;13) Briefly, following lysis of the tissue sections, 50-mer probes specific for the genes of interest were incubated with the samples, forming specific probe-mRNA duplexes, then unhybridized probes were digested by S1 Nuclease, followed by alkaline hydroly
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2013-05-23 12:21:38
正在翻译，请等待...
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2013-05-23 12:23:18
“ ArrayPlate 含量执行了如前所述 ( 12 ; 13 ) 简要地叙述 , 以下 lysis 的组织部分 , 50 mer 探头的特定基因的利益被孵化的样本 , 形成特定的探针的姆尔尼亚双拼别墅 , 然后 unhybridized 探头被消化液 S 1 Nuclease , 其次是碱性水解破坏姆尔尼亚的双工模式
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2013-05-23 12:24:58
ArrayPlate分析用试样执行了作为早先被描述的。(12; 13) 简要地，从事组织部分的病势渐退， 50-mer探针具体为基因利益在套楼公寓孵化了与样品，形成具体探查mRNA套楼公寓，然后unhybridized探针由S1核酸酶消化，跟随由加碱水解毁坏mRNA
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2013-05-23 12:26:38
如上文所述，进行了 ArrayPlate 测定。(12，13)简单地说后裂解的组织切片，感兴趣的基因的特定孵育与样品，形成特异性探针 mRNA 复式公寓，然后由 S1 核酸酶消化 unhybridized 的探针的 50 mer 探头, 紧接着碱性水解摧毁 mRNA 在复式
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2013-05-23 12:28:18
正在翻译，请等待...
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