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  • 匿名
关注:1 2013-05-23 12:21

求翻译:Briefly, following lysis of the tissue sections, 50-mer probes specific for the genes of interest were incubated with the samples, forming specific probe-mRNA duplexes, then unhybridized probes were digested by S1 Nuclease, followed by alkaline hydrolysis to destroy the mRNA in the duplexes是什么意思?

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Briefly, following lysis of the tissue sections, 50-mer probes specific for the genes of interest were incubated with the samples, forming specific probe-mRNA duplexes, then unhybridized probes were digested by S1 Nuclease, followed by alkaline hydrolysis to destroy the mRNA in the duplexes
问题补充:

  • 匿名
2013-05-23 12:21:38
正在翻译,请等待...
  • 匿名
2013-05-23 12:23:18
简言之 , 在 lysis 的组织部分 , 50 mer 探头的特定基因的利益被孵化的样本 , 形成特定的探针的姆尔尼亚双拼别墅 , 然后 unhybridized 探头被消化液 S 1 Nuclease , 其次是碱性水解破坏姆尔尼亚的双工模式
  • 匿名
2013-05-23 12:24:58
简要地,从事组织部分的病势渐退, 50-mer探针具体为基因利益在套楼公寓孵化了与样品,形成具体探查mRNA套楼公寓,然后unhybridized探针由S1核酸酶消化,跟随由加碱水解毁坏mRNA
  • 匿名
2013-05-23 12:26:38
简单地说后裂解的组织切片,感兴趣的基因的特定孵育与样品,形成特异性探针 mRNA 复式公寓,然后由 S1 核酸酶消化 unhybridized 的探针的 50 mer 探头, 紧接着碱性水解摧毁 mRNA 在复式
  • 匿名
2013-05-23 12:28:18
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