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关注：1
2013-05-23 12:21
求翻译：The surface-sterilized seeds were homogenized with a sterile mortar and pestle. Serial dilutions were spread plated on nutrient agar at 30 C for 48 h. Morphologically different colonies were purified by repeated culturing on nutrient agar, identified by Gram-staining and spore staining and then preserved in 20 % glycero是什么意思？ 待解决
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- 离问题结束还有
The surface-sterilized seeds were homogenized with a sterile mortar and pestle. Serial dilutions were spread plated on nutrient agar at 30 C for 48 h. Morphologically different colonies were purified by repeated culturing on nutrient agar, identified by Gram-staining and spore staining and then preserved in 20 % glycero
问题补充： |
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2013-05-23 12:21:38
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2013-05-23 12:23:18
正在翻译，请等待...
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2013-05-23 12:24:58
表面被消炎的种子均匀了与一根不育的灰浆和杵。 在营养琼脂传播了连续稀释镀了在30 C为48 h。 形态上不同的殖民地是purifi编辑通过重覆的开化在营养琼脂， identifi20%丙三醇弄脏然后保存的编辑通过克弄脏和孢子在-80 C。 In addition, some of the surface-sterilized seeds were germinated in sterilized Petri dishes containing two pieces of moist filter paper (Whatman No. 1). The Petri dishes were inc
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2013-05-23 12:26:38
表面消毒的种子是不育的迫击炮和杵匀浆。系列稀释流传镀上营养琼脂在 30 ℃ 为 48 h.形态不同殖民地通过反复培养营养琼脂、 对于由革兰氏染色和染色，然后保存在-80 ℃ 20%甘油的孢子被净化此外，一些表面消毒种子萌发中包含两张湿润 filter 纸 (惠特曼号 1) 的无菌培养皿。培养皿孵育 30 ℃ 5 天了。然后，发芽的种子被压与不育的迫击炮和杵。细菌分离、 净化，种鉴定和保存如上文所述。
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2013-05-23 12:28:18
正在翻译，请等待...
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