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关注：1
2013-05-23 12:21
求翻译：The production of IAA was determined by the colorimetric methodology described by Ribeiro and Cardoso (2012) with some modifications. The isolates were cultured in test tubes containing 3 ml of nutrient broth medium supplemented with 1 g l-1 L-tryptophan at 30 C for 72 h. Cells were harvested by centrifugation at 10,00是什么意思？ 待解决
 悬赏分：1
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The production of IAA was determined by the colorimetric methodology described by Ribeiro and Cardoso (2012) with some modifications. The isolates were cultured in test tubes containing 3 ml of nutrient broth medium supplemented with 1 g l-1 L-tryptophan at 30 C for 72 h. Cells were harvested by centrifugation at 10,00
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2013-05-23 12:21:38
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2013-05-23 12:23:18
正在翻译，请等待...
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2013-05-23 12:24:58
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2013-05-23 12:26:38
生产的 IAA 测定与一些改装的里贝罗和卡多佐 (2012 年) 所述的比色方法。菌株培养液的试管含 3 毫升的营养肉汤培养基以 1 g l-1 L-色氨酸在 30 ℃ 为 72 h.细胞收获用离心法在拥有 10 万 g 为 5 分钟和 100 ll 的上清液加入 96 孔板塑料微孔板与 100 ll Salkowski 试剂混合 (1 毫升的 0.5 mol l 1 FeCl3 和 49 毫升的 35 %hclo4) 为 30 分钟。阅读采取在 530 nm 使用酶标仪 Infinite M200 临 (Tecan，奥地利)。细胞壁主要的介质被用作控制。商业 IAA 用于获取标准曲线方程。
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2013-05-23 12:28:18
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