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关注：1
2013-05-23 12:21
求翻译：Then a 1133 bp P. ostreatus gpd amplicon obtained by PCR amplification with gene specific primers pgEF and pgBR flanking a EcoRI and Bam HI restriction sites was introduced in pMD-E to form the plasmid pMPE.是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
Then a 1133 bp P. ostreatus gpd amplicon obtained by PCR amplification with gene specific primers pgEF and pgBR flanking a EcoRI and Bam HI restriction sites was introduced in pMD-E to form the plasmid pMPE.
问题补充： |
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2013-05-23 12:21:38
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2013-05-23 12:23:18
然后a 1133 bp临ostreatus gpd amplicon获得PCR amplification基因与特定的底漆386和pgBR东边的EcoRI和Bam HI限制站点引入了pMD-E要形成“质粒”（plasmid）pMPE。
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2013-05-23 12:24:58
然后1133 bp P。 ostreatus PCR放大作用获得的gpd侧EcoRI和Bam HI的amplicon与基因具体底漆pgEF和pgBR 制约站点在pMD-E被介绍形成质粒pMPE。
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2013-05-23 12:26:38
然后在偏振模色散-E 形成质粒 pMPE 介绍了扩增 PCR 扩增基因特异性引物 pgEF 和 pgBR 侧翼 EcoRI 和 Bam HI 的限制站点获得的 1133 bp 平菇 gpd 子。
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2013-05-23 12:28:18
正在翻译，请等待...
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