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关注：1
2013-05-23 12:21
求翻译：Subsequently,the amplified hph gene, in which Sph I and Xho I restriction sites were added using primers of hygSF and hygXR (Table 1),was digested and ligated into the EcoRI-Sph I excised vector pMPE to generate the egfp -hph fusion expressing cassette driven by the homologous P. ostreatus gpd promo是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
Subsequently,the amplified hph gene, in which Sph I and Xho I restriction sites were added using primers of hygSF and hygXR (Table 1),was digested and ligated into the EcoRI-Sph I excised vector pMPE to generate the egfp -hph fusion expressing cassette driven by the homologous P. ostreatus gpd promo
问题补充： |
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2013-05-23 12:21:38
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2013-05-23 12:23:18
正在翻译，请等待...
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2013-05-23 12:24:58
随后，被放大的hph基因，在哪些Sph I和Xho I制约站点使用底漆hygSF和hygXR表 (增加1)，是 消化和绑扎入EcoRI-Sph我切除传染媒介pMPE引起egfp - hph融合表达同源驾驶的卡式磁带 P. ostreatus gpd促进者。
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2013-05-23 12:26:38
随后，扩增的 hph 基因，其中报业控股第一和 Xho 我限制站点使用引物 hygSF 和 hygXR (表 1) 添加、 是消化和结扎成 EcoRI Sph 我切除矢量 pMPE 生成 egfp-hph 融合表达同源平菇 gpd 启动子驱动的卡带。
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2013-05-23 12:28:18
正在翻译，请等待...
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