|
关注:1
2013-05-23 12:21
求翻译:Rat ALT1 (rALT1), rALT2or GFP cDNA was subcloned into pAdTrack-CMV(9). The resulting adenoviral vector was digested with PacIand were transfected in HEK293A cells by LipoD 293(Signal Gene, Rockville, MD). Adenovirus-containing supernatants were collected 10 days after transfection to infect fresh HEK293 cells for virus是什么意思?![]() ![]() Rat ALT1 (rALT1), rALT2or GFP cDNA was subcloned into pAdTrack-CMV(9). The resulting adenoviral vector was digested with PacIand were transfected in HEK293A cells by LipoD 293(Signal Gene, Rockville, MD). Adenovirus-containing supernatants were collected 10 days after transfection to infect fresh HEK293 cells for virus
问题补充: |
|
2013-05-23 12:21:38
null
|
|
2013-05-23 12:23:18
Rat ALT 1(参1),参数位或GFP cDNA是到subcloned板定点的CMV(9)。 由此adenoviral vector在消化与被PacIand transfected在嘿咳293A细胞的LipoD 293条基因的信号,Rockville,MD)。 腺病毒含有supernatants被收集后10天至transfection感染新鲜嘿咳293细胞为病毒的放大。 经过3轮的放大、腺病毒制作了大型和净化了中海的梯度ultracentrifugation(9)。
|
|
2013-05-23 12:24:58
正在翻译,请等待...
|
|
2013-05-23 12:26:38
大鼠 ALT1 (rALT1),rALT2or 绿色荧光蛋白基因是极量到 pAdTrack-CMV(9)。由此产生的腺病毒载体消化与 PacIand 在 HEK293A 细胞转染的 LipoD 293 (信号基因,罗克维尔,MD)。腺病毒包含了天花粉蛋白转染感染病毒扩增新鲜 HEK293 细胞后的 10 天。3-4 轮放大后腺病毒在大型和净化的中海集运梯度超速离心法 (9) 被生产。
|
|
2013-05-23 12:28:18
正在翻译,请等待...
|
湖北省互联网违法和不良信息举报平台 | 网上有害信息举报专区 | 电信诈骗举报专区 | 涉历史虚无主义有害信息举报专区 | 涉企侵权举报专区