求翻译：PCR方法的建立，根据等位基因特异性PCR法（AS-PCR）的原理，设计一对特异引物，以含D816V突变的 pEGFP- C1- c-kit重组质粒为模板，通过普通PCR优化退火温度后再优化荧光定量PCR条件。是什么意思？

PCR for the establishment, according to the allele-specific PCR method (AS-PCR) principle, a pair of specific primers to contain D816V mutant pEGFP-C1-c-kit plasmid as a template, by ordinary PCR annealing temperature optimization and then optimize the RT-PCR conditions.

正在翻译，请等待...

正在翻译，请等待...

Establishment of PCR method, according to the allele-specific PCR method (AS-PCR) principle, design specific primer pair, pEGFP-C1-c-kit recombinant plasmid containing D816V mutation as a template by regular PCR optimization optimization of fluorescence quantitative PCR conditions after the annealin

PCR for the establishment, according to the allele-specific PCR method (AS-PCR) principle, a pair of specific primers to contain D816V mutant pEGFP-C1-c-kit plasmid as a template, by ordinary PCR annealing temperature optimization and then optimize the RT-PCR conditions.