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关注:1
2013-05-23 12:21
求翻译:取对数生长期的单层培养细胞,用0.25% Trypsin+0.02% EDTA对单层细胞进行消化、分离成单细胞悬液,进行正常传代、收获细胞做相关实验是什么意思? 待解决
悬赏分:1
- 离问题结束还有
取对数生长期的单层培养细胞,用0.25% Trypsin+0.02% EDTA对单层细胞进行消化、分离成单细胞悬液,进行正常传代、收获细胞做相关实验
问题补充: |
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2013-05-23 12:21:38
Logarithmic growth phase of the cell monolayer culture with 0.25% Trypsin +0.02% EDTA digestion of single cells, separated into single cell suspension, the normal passage, cells were harvested to do experiments
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2013-05-23 12:23:18
anagen 20,482 from the single-level cell culture, with 0.25 % + 0.02 % Trypsin EDTA digestion of single-layer cells, separated into a single-cell suspension liquid, in the course of their normal generational related experiment done, harvesting stem cells
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2013-05-23 12:24:58
Takes the logarithm vegetal period the monolayer culture cell, carries on the digestion with 0.25% Trypsin+0.02% EDTA to the single-layer cell, separates the single cell to hang the fluid, carries on the normal transfer of generation, the harvest cell does the correlation experiment
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2013-05-23 12:26:38
Preparation of monolayer culture of logarithmic growth phase cells, digestion, with 0.25%Trypsin+0.02%EDTA single layer of cells into single cells suspensions for normal passage, harvesting cells as related experiments
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2013-05-23 12:28:18
Logarithmic growth phase of the cell monolayer culture with 0.25% Trypsin +0.02% EDTA digestion of single cells, separated into single cell suspension, the normal passage, cells were harvested to do experiments
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