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关注:1
2013-05-23 12:21
求翻译:Reaction products were separated by agarose gel electrophoresis by adding 1 μl of 6X loading dye to reaction mixture and loading 6 μl onto a 1.5% agarose gel. The buffer in the electrophoresis chamber and in the agarose gel was TAE 1X and contained 2 μl Sibergreen per 100 ml gel. 80 volts were applied across the gel. F是什么意思? 待解决
悬赏分:1
- 离问题结束还有
Reaction products were separated by agarose gel electrophoresis by adding 1 μl of 6X loading dye to reaction mixture and loading 6 μl onto a 1.5% agarose gel. The buffer in the electrophoresis chamber and in the agarose gel was TAE 1X and contained 2 μl Sibergreen per 100 ml gel. 80 volts were applied across the gel. F
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2013-05-23 12:21:38
反应混合物中加入1μL6X上样染料和装载到1.5%琼脂糖凝胶6μL反应产物琼脂糖凝胶电泳分离。在琼脂糖凝胶电泳室和缓冲栓塞1X和2μLSibergreen每100毫升凝胶。适用于整个凝胶80伏。最后凝胶是由凝胶暴露在紫外线照射下的可视化和被拍到。被列入每个琼脂糖凝胶电泳DNA标准分子量大小标记。
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2013-05-23 12:23:18
产品的反应是分开agarose电泳凝胶体,加入1μl6X装载的染料,反应混合物和装载6μl上有1.5%agarose凝胶。 在缓冲区的电泳分庭和在agarose凝胶是泰1x和2所载每100毫升μl sibergreen凝胶。 80伏特均全面适用的凝胶。 最后的凝胶是所顾虑的凝胶揭露,紫外线灯和拍了照。 DNA分子重量标准尺寸标记是包括在每一agarose凝胶电泳运行。
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2013-05-23 12:24:58
反应产品由琼脂糖胶凝
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2013-05-23 12:26:38
反应产物的琼脂糖凝胶电泳分隔添加的加载到反应混合物的染料和加载到 1.5%琼脂糖凝胶上的 6 μl X 6 1 μl。缓冲区和琼脂糖凝胶电泳室泰 1 X,包含 2 μl Sibergreen 每 100 毫升凝胶。凝胶跨应用了 80 伏特。最后凝胶通过公开紫外光凝胶可视化和被拍到。每个运行的琼脂糖凝胶电泳中列入了 DNA 分子的标准体重大小标记。
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2013-05-23 12:28:18
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