|
关注:1
2013-05-23 12:21
求翻译:DNA was extracted by centrifugation of 1.5 ml of resuspension solution in Eppendrof tube at 10000 rpm for 5 min and removal of the supernatant. A volume of 800 μl of lyses buffer solution (5 mol NaCl, 100 mM Tris-base, 20 mM EDTA-Na2 and CTAB 20%) were added and the samples were vortexed.是什么意思? 待解决
悬赏分:1
- 离问题结束还有
DNA was extracted by centrifugation of 1.5 ml of resuspension solution in Eppendrof tube at 10000 rpm for 5 min and removal of the supernatant. A volume of 800 μl of lyses buffer solution (5 mol NaCl, 100 mM Tris-base, 20 mM EDTA-Na2 and CTAB 20%) were added and the samples were vortexed.
问题补充: |
|
2013-05-23 12:21:38
DNA的提取Eppendrof管的再悬浮溶液1.5毫升离心5分钟,去除上清10000转。分别加入800μl裂解缓冲液(5摩尔氯化钠,三基地,100毫米20毫米EDTA - NA2和CTAB 20%)的量和样品振荡。
|
|
2013-05-23 12:23:18
DNA是由微离心机的重新悬浮对1.5ml的解决办法在eppendrof管在10000RPM的5分钟和清除等蛋白质进行回收的。 第一卷的缓冲区的800个μl解决(5mol氯化钠NaCl,100mm Tris-基地,20mm EDTA-Na2和ctab20%)增加了vortexed,抽取的样本。
|
|
2013-05-23 12:24:58
正在翻译,请等待...
|
|
2013-05-23 12:26:38
DNA 是由离心法的上清液脱 1.5 毫升的再悬浮液在 5 分钟 10000 rpm Eppendrof 管中提取的。800 Μl lyses 添加了缓冲溶液 (5 mol 氯化钠、 100 毫米席基地、 20 毫米 EDTA Na2 和 CTAB 20%) 的量和样品是 vortexed。
|
|
2013-05-23 12:28:18
null
|
湖北省互联网违法和不良信息举报平台 | 网上有害信息举报专区 | 电信诈骗举报专区 | 涉历史虚无主义有害信息举报专区 | 涉企侵权举报专区