求翻译：以引物MnF1、 MnR1扩增得到manA1；以引物MnF2、MnR1扩增得到manA2(含信号肽)。在基因manA2基础上，重新设计合成下游引物MnR2，以引物MnF2、MnR2扩增得到manA2a(含His-Tag)。是什么意思？

With primers mnf1, mnr1 amplified mana1; with primers mnf2, mnr1 amplified mana2 (including signal peptide). On the basis of gene mana2, re-design and synthesis of the downstream primer mnr2, with primers mnf2, mnr2 amplified mana2a (including his-tag).

The gfm MnF MnR 1 , 1 1 expansion; to lead the manA MnF MnR 2 , 1 2 manA expanded access (including signal peptide). In the case of genetic manA 2, re-design based on synthetic downstream primer MnR MnF 2 2, in order to lead, MnR manA 2A by 2 expansion (included His - Tag).

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Primer amplification are of MnF1, MnR1, manA1; to amplification primers MnF2, MnR1 are manA2 (containing signal peptide). ManA2, based on synthesis downstream primers MnR2 redesign, to amplification primers MnF2, MnR2 are manA2a (including His-Tag).