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关注:1
2013-05-23 12:21
求翻译:Western Blot采用的是聚丙烯酰胺凝胶电泳,被检测物是蛋白质,“探针”是抗体,“显色”用标记的二抗。经过PAGE分离的蛋白质样品,转移到固相载体(例如硝酸纤维素薄膜)上,固相载体以非共价键形式吸附蛋白质,且能保持电泳分离的多肽类型及其生物学活性不变。以固相载体上的蛋白质或多肽作为抗原,与对应的抗体起免疫反应,再与酶或同位素标记的第二抗体起反应,经过底物显色或放射自显影以检测电泳分离的特异性目的基因表达的蛋白成分。该技术也广泛应用于检测蛋白水平的表达。是什么意思? 待解决
悬赏分:1
- 离问题结束还有
Western Blot采用的是聚丙烯酰胺凝胶电泳,被检测物是蛋白质,“探针”是抗体,“显色”用标记的二抗。经过PAGE分离的蛋白质样品,转移到固相载体(例如硝酸纤维素薄膜)上,固相载体以非共价键形式吸附蛋白质,且能保持电泳分离的多肽类型及其生物学活性不变。以固相载体上的蛋白质或多肽作为抗原,与对应的抗体起免疫反应,再与酶或同位素标记的第二抗体起反应,经过底物显色或放射自显影以检测电泳分离的特异性目的基因表达的蛋白成分。该技术也广泛应用于检测蛋白水平的表达。
问题补充: |
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2013-05-23 12:21:38
正在翻译,请等待...
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2013-05-23 12:23:18
Western Blot used the polyacrylamide gel electrophoresis, protein is detected, The property is "needle" antibodies, and "color" of markings used 2 anti-. As a result of separation PAGE protein samples, and transferred to the fixed-carrier (for example, cellulose nitrate film) on solid carriers to no
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2013-05-23 12:24:58
Western Blot uses is the polyacrylamide gel electrophoresis, by the test item is the protein, “the probe” is the immune body, “the coloration” uses the mark two anti-.After the PAGE separation protein sample, shifts to the solid phase carrier (e.g. nitrocellulose thin film) on, the solid phase carri
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2013-05-23 12:26:38
Western Blot using polyacrylamide gel electrophoresis, detection is a proteins, "probe" is the antibody, "colour" with Mark II anti-. After PAGE separation of protein samples transferred to the solid phase carrier (such as nitrocellulose film), the solid phase carrier proteins in the form of non-Cov
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2013-05-23 12:28:18
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