求翻译：为了确定已克隆的辣椒多聚半乳糖醛酸酶基因(CaPG)在辣椒耐贮性等方面所起的作用,本试验拟在获得该基因全长cDNA序列的基础上，构建该基因由组成型启动子CaMV35S超量表达载体，为植物材料遗传转化的方法鉴定该基因的功能做准备。方法：将CaPG全长基因的PCR产物回收后，按一定比例与克隆载体连接，将连接产物转入到大肠杆菌DH5α，培养检测。将含有目的基因的重组质粒经双酶切，同时将表达载体用相同的限制性内切酶进行酶切，然后利用T4DNA连接酶连接，得到重组质粒。转化大肠杆菌DH5α，筛选重组子。提取阳性克隆质粒，经PCR方法检测得到重组克隆载体。是什么意思？

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In order to determine already clones because the hot pepper gathers the galacturonic acid substrat (CaPG) the role which in aspects and so on hot pepper keeping quality plays, this experiment draws up in obtains this gene span cDNA sequence in the foundation, constructs this gene by to be composed t

In order to determine the clone of pepper poly galacturonic acid lipase gene (CaPG) storability, and so the role of pepper, this test to be obtained on the basis of full length cDNA sequence of the gene, build by the constitutive promoter of the gene expression of CaMV35S excess carriers, for the id