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  • 匿名
关注:1 2013-05-23 12:21

求翻译:取一个癌细胞及其正常细胞对照体,用轻试剂标记癌细胞蛋白。用重试剂标记另一个正常细胞蛋白。这两种细胞在等量混合后,纯化蛋白质并用胰蛋白酶消化,用亲和素柱纯化作了标记的半胱氨酸残基的肽。是什么意思?

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取一个癌细胞及其正常细胞对照体,用轻试剂标记癌细胞蛋白。用重试剂标记另一个正常细胞蛋白。这两种细胞在等量混合后,纯化蛋白质并用胰蛋白酶消化,用亲和素柱纯化作了标记的半胱氨酸残基的肽。
问题补充:

  • 匿名
2013-05-23 12:21:38
Take a cancer cell and its normal cells control the body, the cancer cell proteins marked with a light reagent. Heavy reagent mark another normal cell protein. These two cells in the same amount of mixture, the purified protein digested with trypsin, using the avidin column was labeled cysteine ​​re
  • 匿名
2013-05-23 12:23:18
A cancer cells and normal cells in the body, cancer cells marked with light reagents protein. In the reagent mark another normal cell protein. These two types of cells, such as the purification of proteins after mixing, and trypsin digested, and the parent, and of the Mark column purification for th
  • 匿名
2013-05-23 12:24:58
Takes a cancer cell and the normal cell comparison body, marks the cancer cell protein with the light reagent.Marks another normal cell protein with the heavy reagent.These two kind of cells after the isometric mix, the purification protein and with the trypsin digestion, with kissed have made the m
  • 匿名
2013-05-23 12:26:38
Take control of a cancer cell and a normal cell, marked with a light Kit cancer cell protein. Marked with the reagent and the other normal cell protein. After mixing the two cells in the same amount, purification and trypsin digestion of protein, marked with the effect of affinity column purificatio
  • 匿名
2013-05-23 12:28:18
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