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关注:1 2013-05-23 12:21

求翻译:1. 5 MTT实验 制备细胞悬液,调整细胞浓度为1×105,实验分成药物处理组:全反式维甲酸(用二甲基亚砜配制)1,10,100,1000nmol/L组;紫杉醇(用无水乙醇配制)1,10,100,1000nmol/L组;无水乙醇组(无水乙醇和培养基);二甲基亚砜组(二甲基亚砜和培养基)共10组,和阴性对照组(培养基),每组设6个孔。根据各药物浓度的结果,进一步分成全反式维甲酸和紫杉醇联合应用组。取96孔培养板,每孔加入上述浓度的细胞悬液100ul,按以上分组进行实验,将紫杉醇和全反式维甲酸用DMEM培养液稀释,使终浓度分别为:1,10,100,1 000nmol/L,将盛有细胞悬液和上述药物的96孔板放置在37是什么意思?

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1. 5 MTT实验 制备细胞悬液,调整细胞浓度为1×105,实验分成药物处理组:全反式维甲酸(用二甲基亚砜配制)1,10,100,1000nmol/L组;紫杉醇(用无水乙醇配制)1,10,100,1000nmol/L组;无水乙醇组(无水乙醇和培养基);二甲基亚砜组(二甲基亚砜和培养基)共10组,和阴性对照组(培养基),每组设6个孔。根据各药物浓度的结果,进一步分成全反式维甲酸和紫杉醇联合应用组。取96孔培养板,每孔加入上述浓度的细胞悬液100ul,按以上分组进行实验,将紫杉醇和全反式维甲酸用DMEM培养液稀释,使终浓度分别为:1,10,100,1 000nmol/L,将盛有细胞悬液和上述药物的96孔板放置在37
问题补充:

  • 匿名
2013-05-23 12:26:38
1.5 MTT experimental preparation cell suspended liquid, adjustment cell concentration for 1x105, experimental into drug processing Group: full anti-type dimension formic acid (with II methyl Asia sulfone preparation) 1,10,100,1000nmol/L Group; yew alcohol (with no water ethanol preparation) 1,10,100,1000nmol/L group; no water ethanol Group (no water ethanol and medium); II methyl jiya sulfone Group (II methyl Asia sulfone and medium) total 10 group, and negative controlled group (medium), each group set 6 a hole. Depending on the concentration results, further divided into all-TRANS Retinoic acid and paclitaxel combined with application groups. Take the 96-well culture plates, each hole to join the above concentration cell suspension 100ul, grouped by more than experimental, paclitaxel and all-TRANS Retinoic acid diluted with DMEM culture fluid, concentration, respectively: 1,10,100,1 000nmol/L, containing cell suspension and 96-well plate placed above drug cultivation in 37
 
 
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