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  • 匿名
关注:1 2013-05-23 12:21

求翻译:本实验成功培养了S-D大鼠血管内皮细胞。国内外报道,组织块贴块法和胶原酶消化法均可以在体外培养内皮细胞,贴块法较容易,但周期较长,细胞不纯,常混有成纤维细胞;消化法相对较难,但细胞易成活,所需周期较短,纯度较高。消化法培养内皮细胞成功的关键是掌握好消化时间,一般在15~20min,另外一定注意无菌操作,否则会前功尽弃。是什么意思?

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本实验成功培养了S-D大鼠血管内皮细胞。国内外报道,组织块贴块法和胶原酶消化法均可以在体外培养内皮细胞,贴块法较容易,但周期较长,细胞不纯,常混有成纤维细胞;消化法相对较难,但细胞易成活,所需周期较短,纯度较高。消化法培养内皮细胞成功的关键是掌握好消化时间,一般在15~20min,另外一定注意无菌操作,否则会前功尽弃。
问题补充:

  • 匿名
2013-05-23 12:21:38
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  • 匿名
2013-05-23 12:23:18
正在翻译,请等待...
  • 匿名
2013-05-23 12:24:58
This tested has successfully raised the S-D big mouse blood vessel endothelial cell.The domestic and foreign reports, the organization block pastes the block law and the collagenase digestion law may in vitro raise endothelial cell, pastes the block law to be easy, but the cycle is long, cell impure
  • 匿名
2013-05-23 12:26:38
This experiment is successful S-D of rat vascular endothelial cells. Reported at home and abroad, organizations can paste method and collagenase digestion in endothelial cells in vitro, posted by block easier, but cycles are longer, cells are not pure, often mixed fiber cell; relatively difficult di
 
 
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