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关注：1
2013-05-23 12:21
求翻译：A TSK-Gel Super ODS column (Tosoh Corporation) will be maintained at room temperature. Gradient solvents of 0.1% trifluoroacetic acid in HPLC grade water (Solvent A), 0.1% trifluroacetic acid in acetonitrile (Solvent B) and 100% methanol (Solvent C) will be used to elute the sample at a flow rate of 1.0 ml per minute. Methanol will be used as a blank and to flush the column between runs. Total run time is 37 minutes. The initial solvent composition is 0% solvent A and 100% solvent B. A linear gradient will be used to increase solvent A from 0% to 10% within 7 minutes. This solvent composition will be maintained at an isocratic flow for 3 minutes. Solvent A will then be increased from 0% to 40% using a 20 minute linear gradient. This composition will be maintained for 2 minutes and returned to the initial composition in 3 minutes. A sample injection of 20 是什么意思？ 待解决
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A TSK-Gel Super ODS column (Tosoh Corporation) will be maintained at room temperature. Gradient solvents of 0.1% trifluoroacetic acid in HPLC grade water (Solvent A), 0.1% trifluroacetic acid in acetonitrile (Solvent B) and 100% methanol (Solvent C) will be used to elute the sample at a flow rate of 1.0 ml per minute. Methanol will be used as a blank and to flush the column between runs. Total run time is 37 minutes. The initial solvent composition is 0% solvent A and 100% solvent B. A linear gradient will be used to increase solvent A from 0% to 10% within 7 minutes. This solvent composition will be maintained at an isocratic flow for 3 minutes. Solvent A will then be increased from 0% to 40% using a 20 minute linear gradient. This composition will be maintained for 2 minutes and returned to the initial composition in 3 minutes. A sample injection of 20
问题补充： |
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2013-05-23 12:26:38
TSK 凝胶超级 ODS 列 (Tosoh) 将维持在室温。0.1%氟乙酸高效液相色谱法级水 （溶剂 A），在乙腈 (溶剂 B) 和 (溶剂 C) 100%甲醇 0.1 %trifluroacetic 酸中的渐变溶剂将用于析出流速度每分钟 1.0 ml 的示例。将用于甲醇，为空白，刷新列之间运行。总运行的时间为 37 分钟。初始的溶剂成分是溶剂 A 0%和 100%溶剂 B.线性渐变将用于在 7 分钟内提高溶剂 A 从 0%到 10%。这种溶剂的成分将保持在熊胆流 3 分钟。溶剂 A 然后将从 0%增加到 40%使用 20 分钟的线性渐变。这篇作文将保持 2 分钟，并返回到初始组成 3 分钟。高效液相色谱法分析，将注入 20
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