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关注：1
2013-05-23 12:21
求翻译：TEM observation of the chloroplast ultrastructure of soybean seedling was performed according to the previous methods (Helliot et al., 2003). Fresh leaves were sliced into 1.5 2 mm. After fixation with 3.5% glutaraldehyde for 24 h, leaf cells were post-fixed with 1% osmic acid at 4 C for 4 h and then were dehydrated是什么意思？ 待解决
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TEM observation of the chloroplast ultrastructure of soybean seedling was performed according to the previous methods (Helliot et al., 2003). Fresh leaves were sliced into 1.5 2 mm. After fixation with 3.5% glutaraldehyde for 24 h, leaf cells were post-fixed with 1% osmic acid at 4 C for 4 h and then were dehydrated
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2013-05-23 12:21:38
TEM观察大豆幼苗叶绿体超微结构的是按照以前的方法（ Helliot等人， 2003 ）进行。
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2013-05-23 12:23:18
正在翻译，请等待...
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2013-05-23 12:24:58
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2013-05-23 12:26:38
按照以前的方法 (Helliot et al.，2003年） 进行了大豆幼苗叶绿体超微结构的透射电镜观察。新鲜叶子被切掉进 1.5 2 毫米。与 24 h 的 3.5%戊二醛固定后, 叶细胞被后固定 1 %osmic 酸 4 c 4 h，然后被用乙醇脱水。然后该示例嵌入在中准备 100%的新鲜 Epon 812 和聚合在 24 h 80 C。超微结构观测获得的样本被切开了作为超薄切片 (60 毫微米) LKB ultramicrotome 上。然后超薄切片采摘后 250-网格，并被后沾有铀酰醋酸盐和铅柠檬酸。最后，用透射电镜 (H-600-A-2、 日立、 日本) 获得了透射电子显微镜图像。
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2013-05-23 12:28:18
正在翻译，请等待...
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