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关注:1
2013-05-23 12:21
求翻译:The cloned genes encoding the circularly permuted 1,3–1,4-b-glucanase cpMAC-57 and wtXYN were fused by splicing-by-overlap extension .The conditions for performing PCR were as described earlier . In separate primary amplifications (see Fig. 1), three overlapping DNA fragments, A, B, and C, were amplified by PCR.是什么意思? 待解决
悬赏分:1
- 离问题结束还有
The cloned genes encoding the circularly permuted 1,3–1,4-b-glucanase cpMAC-57 and wtXYN were fused by splicing-by-overlap extension .The conditions for performing PCR were as described earlier . In separate primary amplifications (see Fig. 1), three overlapping DNA fragments, A, B, and C, were amplified by PCR.
问题补充: |
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2013-05-23 12:21:38
克隆的基因编码的环状变序的1,3-1,4 -B-葡聚糖cpMAC -57和wtXYN被剪接逐重叠延伸.The条件稠用于进行PCR的如前所述。
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2013-05-23 12:23:18
输入圆地被交换的1,3-1,4-b-glucanase cpMAC-57和wtXYN的被克隆的基因由接合由交叠引伸熔化。执行PCR的条件是如所描述前。在分开的主要放大作用(参见图1),三个重叠的脱氧核糖核酸片段, A, B,并且C,由PCR放大。
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2013-05-23 12:24:58
输入圆地被交换的1,3-1,4-b-glucanase cpMAC-57和wtXYN的被克隆的基因熔化由接合由重叠引伸。条件为执行的PCR是如所描述及早。 在分开的主要放大作用 (看见。 1个),三个重叠的脱氧核糖核酸片段, A、B和C,由PCR放大。
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2013-05-23 12:26:38
克隆的基因编码的循环排列后 1,3-1,4-b-葡聚糖酶 cpMAC-57 和 wtXYN 进行融合的拼接的重叠延伸。用于执行聚合酶链反应条件如前面所述。在分离主扩增 (见图 1),三个重叠的 DNA 片段,A、 B 和 C,pcr 扩增。
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2013-05-23 12:28:18
将其编码的被克隆的基因圆形地改变 1, 3-1, 4-b-glucanase 的 cpMAC-57 和 wtXYN 的序列 被连接处被重叠的扩大熔合用于实行 PCR 的 .The 条件根据说明是更早的。在单独最初放大中 ( 看见无花果。1),三与人互搭 DNA 破碎, A, B, C,被 PCR 扩大。
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